## ---- echo=FALSE, results="hide", warning=FALSE--------------------------
suppressPackageStartupMessages({
    library(trackViewer)
    library(rtracklayer)
    library(Gviz)
    library(TxDb.Hsapiens.UCSC.hg19.knownGene)
    library(org.Hs.eg.db)
    library(VariantAnnotation)
})
knitr::opts_chunk$set(warning=FALSE, message=FALSE)

## ----plotComp,echo=TRUE,fig.keep='none'----------------------------------
library(Gviz)
library(rtracklayer)
library(trackViewer)
extdata <- system.file("extdata", package="trackViewer",
                       mustWork=TRUE)
gr <- GRanges("chr11", IRanges(122929275, 122930122), strand="-")
fox2 <- importScore(file.path(extdata, "fox2.bed"), format="BED",
                    ranges=gr)
fox2$dat <- coverageGR(fox2$dat)

viewTracks(trackList(fox2), gr=gr, autoOptimizeStyle=TRUE, newpage=FALSE)

dt <- DataTrack(range=fox2$dat[strand(fox2$dat)=="-"] , 
                genome="hg19", type="hist", name="fox2", 
                window=-1, chromosome="chr11", 
                fill.histogram="black", col.histogram="NA",
                background.title="white",
                col.frame="white", col.axis="black",
                col="black", col.title="black")
plotTracks(dt, from=122929275, to=122930122, strand="-")

## ----Gviz,echo=FALSE,fig.cap='Plot data with **Gviz** and **trackViewer**. Note that **trackViewer** can generate similar figure as **Gviz** with several lines of simple codes.',fig.width=6,fig.height=1.5----
viewerStyle <- trackViewerStyle()
setTrackViewerStyleParam(viewerStyle, "margin", c(.01, .13, .02, .02))
empty <- DataTrack(showAxis=FALSE, showTitle=FALSE, background.title="white")
plotTracks(list(empty, dt), from=122929275, to=122930122, strand="-")
pushViewport(viewport(0, .5, 1, .5, just=c(0, 0)))
viewTracks(trackList(fox2), viewerStyle=viewerStyle, 
                 gr=gr, autoOptimizeStyle=TRUE, newpage=FALSE)
popViewport()
grid.text(label="Gviz track", x=.3, y=.4)
grid.text(label="trackViewer track", x=.3, y=.9)

## ----lostcode,echo=TRUE,fig.keep='none'----------------------------------
gr <- GRanges("chr1", IRanges(c(1, 6, 10), c(3, 6, 12)), score=c(3, 4, 1))
dt <- DataTrack(range=gr, data="score", type="hist")
plotTracks(dt, from=2, to=11)
tr <- new("track", dat=gr, type="data", format="BED")
viewTracks(trackList(tr), chromosome="chr1", start=2, end=11)

## ----GvizLost,echo=FALSE,fig.cap='Plot data with **Gviz** and **trackViewer**. Note that **trackViewer** is not only including more details but also showing all the data involved in the given range.',fig.width=6,fig.height=1.5----
plotTracks(list(empty, dt), from=2, to=11)
pushViewport(viewport(0, .5, 1, .5, just=c(0, 0)))
viewTracks(trackList(tr), viewerStyle=viewerStyle, 
           chromosome="chr1", start=2, end=11, 
           autoOptimizeStyle=TRUE, newpage=FALSE)
popViewport()
grid.text(label="Gviz track", x=.3, y=.4)
grid.text(label="trackViewer track", x=.3, y=.9)

## ----importData----------------------------------------------------------
library(trackViewer)
extdata <- system.file("extdata", package="trackViewer",
                       mustWork=TRUE)
repA <- importScore(file.path(extdata, "cpsf160.repA_-.wig"),
                    file.path(extdata, "cpsf160.repA_+.wig"),
                    format="WIG")
## because the wig file does not contain strand info, 
## we need to set it manually
strand(repA$dat) <- "-"
strand(repA$dat2) <- "+"

## ----coverage------------------------------------------------------------
fox2 <- importScore(file.path(extdata, "fox2.bed"), format="BED",
                    ranges=GRanges("chr11", IRanges(122929000, 122931000)))
dat <- coverageGR(fox2$dat)
## we can split the data by strand into two different track channels
## here we set the dat2 slot to save the negative strand info, 
## reverse order as previous. 
fox2$dat <- dat[strand(dat)=="+"]
fox2$dat2 <- dat[strand(dat)=="-"]

## ----geneModel-----------------------------------------------------------
library(TxDb.Hsapiens.UCSC.hg19.knownGene)
library(org.Hs.eg.db)
gr <- GRanges("chr11", IRanges(122929275, 122930122), strand="-")
trs <- geneModelFromTxdb(TxDb.Hsapiens.UCSC.hg19.knownGene,
                         org.Hs.eg.db,
                         gr=gr)

## ----viewTracks,fig.cap='plot data and annotation information along genomic coordinates',fig.width=6,fig.height=1.5----
viewerStyle <- trackViewerStyle()
setTrackViewerStyleParam(viewerStyle, "margin", c(.1, .05, .02, .02))
vp <- viewTracks(trackList(repA, fox2, trs), 
                 gr=gr, viewerStyle=viewerStyle, 
                 autoOptimizeStyle=TRUE)
addGuideLine(c(122929767, 122929969), vp=vp)
addArrowMark(list(x=122929650, 
                  y=2), # 2 means track 2 from bottom.
             label="label",
             col="blue",
             vp=vp)

## ----optSty--------------------------------------------------------------
optSty <- optimizeStyle(trackList(repA, fox2, trs))
trackList <- optSty$tracks
viewerStyle <- optSty$style

## ----viewTracksXaxis,fig.cap='plot data with x-scale',fig.width=6,fig.height=1.5----
setTrackViewerStyleParam(viewerStyle, "xaxis", FALSE)
setTrackViewerStyleParam(viewerStyle, "margin", c(.01, .05, .01, .01))
setTrackXscaleParam(trackList[[1]], "draw", TRUE)
setTrackXscaleParam(trackList[[1]], "gp", list(cex=.5))
viewTracks(trackList, gr=gr, viewerStyle=viewerStyle)

## ----viewTracksYaxis,fig.cap='plot data with y-axis in right side',fig.width=6,fig.height=1.5----
setTrackViewerStyleParam(viewerStyle, "margin", c(.01, .05, .01, .05))
for(i in 1:2){
    setTrackYaxisParam(trackList[[i]], "main", FALSE)
}
## adjust y scale
setTrackStyleParam(trackList[[1]], "ylim", c(0, 25))
setTrackStyleParam(trackList[[2]], "ylim", c(-25, 0))
viewTracks(trackList, gr=gr, viewerStyle=viewerStyle)

## ----viewTracksYlab,fig.cap='plot data with adjusted color and size of y label',fig.width=6,fig.height=1.5----
setTrackStyleParam(trackList[[1]], "ylabgp", list(cex=.8, col="green"))
## set cex to avoid automatic adjust
setTrackStyleParam(trackList[[2]], "ylabgp", list(cex=.8, col="blue"))
setTrackStyleParam(trackList[[2]], "marginBottom", .2)
viewTracks(trackList, gr=gr, viewerStyle=viewerStyle)

## ----viewTracksYlabTopBottom,fig.cap='plot data with adjusted y label position',fig.width=6,fig.height=1.5----
setTrackStyleParam(trackList[[1]], "ylabpos", "bottomleft")
setTrackStyleParam(trackList[[2]], "ylabpos", "topright")
setTrackStyleParam(trackList[[2]], "marginTop", .2)
viewTracks(trackList, gr=gr, viewerStyle=viewerStyle)

## ----viewTracksYlabUpsDown,fig.cap='plot data with adjusted transcripts name position',fig.width=6,fig.height=1.5----
trackListN <- trackList
setTrackStyleParam(trackListN[[3]], "ylabpos", "upstream")
setTrackStyleParam(trackListN[[4]], "ylabpos", "downstream")
## set cex to avoid automatic adjust
setTrackStyleParam(trackListN[[3]], "ylabgp", list(cex=.6))
setTrackStyleParam(trackListN[[4]], "ylabgp", list(cex=.6))
gr1 <- range(unlist(GRangesList(sapply(trs, function(.ele) .ele$dat))))
start(gr1) <- start(gr1) - 2000
end(gr1) <- end(gr1) + 2000
viewTracks(trackListN, gr=gr1, viewerStyle=viewerStyle)

## ----viewTracksCol,fig.cap='plot data with adjusted track color',fig.width=6,fig.height=1.5----
setTrackStyleParam(trackList[[1]], "color", c("green", "black"))
setTrackStyleParam(trackList[[2]], "color", c("black", "blue"))
for(i in 3:length(trackList)) 
    setTrackStyleParam(trackList[[i]], "color", "black")
viewTracks(trackList, gr=gr, viewerStyle=viewerStyle)

## ----viewTracksHeight,fig.cap='plot data with adjusted track height',fig.width=6,fig.height=1.5----
trackListH <- trackList
setTrackStyleParam(trackListH[[1]], "height", .1)
setTrackStyleParam(trackListH[[2]], "height", .44)
for(i in 3:length(trackListH)){
    setTrackStyleParam(trackListH[[i]], "height", 
                       (1-(0.1+0.44))/(length(trackListH)-2))
}
viewTracks(trackListH, gr=gr, viewerStyle=viewerStyle)

## ----viewTracksNames,fig.cap='change the track names',fig.width=6,fig.height=1.5----
names(trackList) <- c("cpsf160", "fox2", rep("HSPA8", 5))
viewTracks(trackList, gr=gr, viewerStyle=viewerStyle)

## ----viewTracksPaired,fig.cap='show two data in the same track',fig.width=6,fig.height=1.2----
cpsf160 <- importScore(file.path(extdata, "cpsf160.repA_-.wig"),
                       file.path(extdata, "cpsf160.repB_-.wig"),
                       format="WIG")
strand(cpsf160$dat) <- strand(cpsf160$dat2) <- "-"
setTrackStyleParam(cpsf160, "color", c("black", "red"))
viewTracks(trackList(trs, cpsf160), gr=gr, viewerStyle=viewerStyle)

## ----viewTracksFlipped,fig.cap='show data in the flipped track',fig.width=6,fig.height=2----
viewerStyleF <- viewerStyle
setTrackViewerStyleParam(viewerStyleF, "flip", TRUE)
setTrackViewerStyleParam(viewerStyleF, "xaxis", TRUE)
setTrackViewerStyleParam(viewerStyleF, "margin", c(.1, .05, .01, .01))
vp <- viewTracks(trackList, gr=gr, viewerStyle=viewerStyleF)
addGuideLine(c(122929767, 122929969), vp=vp)
addArrowMark(list(x=122929650,
                  y=2),
             label="label",
             col="blue",
             vp=vp)

## ----theme_bw,fig.cap='theme_bw',fig.width=6,fig.height=2----------------
optSty <- optimizeStyle(trackList(repA, fox2, trs), theme="bw")
trackList <- optSty$tracks
viewerStyle <- optSty$style
vp <- viewTracks(trackList, gr=gr, viewerStyle=viewerStyle)

## ----theme_col,fig.cap='theme_col',fig.width=6,fig.height=2--------------
optSty <- optimizeStyle(trackList(repA, fox2, trs), theme="col")
trackList <- optSty$tracks
viewerStyle <- optSty$style
vp <- viewTracks(trackList, gr=gr, viewerStyle=viewerStyle)

## ----axis.break,fig.cap='axis.break',fig.width=6,fig.height=2------------
gr.breaks <- GRanges("chr11", 
                     IRanges(c(122929275, 122929575, 122929775), 
                             c(122929555, 122929725, 122930122)), 
                     strand="-", percentage=c(.4, .2, .4))
vp <- viewTracks(trackList, gr=gr.breaks, viewerStyle=viewerStyle)

## ----viewTracksOperator1,fig.cap='show data with operator "+"',fig.width=6,fig.height=2----
newtrack <- repA
## must keep same format for dat and dat2
newtrack <- parseWIG(newtrack, "chr11", 122929275, 122930122)
newtrack$dat2 <- newtrack$dat
newtrack$dat <- fox2$dat2
setTrackStyleParam(newtrack, "color", c("blue", "red"))
viewTracks(trackList(newtrack, trs), 
           gr=gr, viewerStyle=viewerStyle, operator="+")

## ----viewTracksOperator2,fig.cap='show data with operator "-"',fig.width=6,fig.height=2----
viewTracks(trackList(newtrack, trs), gr=gr, viewerStyle=viewerStyle, operator="-")

## ----viewTracksOperator3,fig.cap='show data with operator "-"',fig.width=6,fig.height=2----
newtrack$dat <- GRoperator(newtrack$dat, newtrack$dat2, col="score", operator="-")
newtrack$dat2 <- GRanges()
viewTracks(trackList(newtrack, trs), gr=gr, viewerStyle=viewerStyle)

## ----lolliplot1, fig.width=6, fig.height=3-------------------------------
SNP <- c(10, 12, 1400, 1402)
sample.gr <- GRanges("chr1", IRanges(SNP, width=1, names=paste0("snp", SNP)))
features <- GRanges("chr1", IRanges(c(1, 501, 1001), 
                                    width=c(120, 400, 405),
                                    names=paste0("block", 1:3)))
lolliplot(sample.gr, features)
## more SNPs
SNP <- c(10, 100, 105, 108, 400, 410, 420, 600, 700, 805, 840, 1400, 1402)
sample.gr <- GRanges("chr1", IRanges(SNP, width=1, names=paste0("snp", SNP)))
lolliplot(sample.gr, features)
## define the range
lolliplot(sample.gr, features, ranges = GRanges("chr1", IRanges(104, 109)))

## ----lolliplot2, fig.width=6, fig.height=3-------------------------------
features$fill <- c("#FF8833", "#51C6E6", "#DFA32D")
lolliplot(sample.gr, features)

## ----lolliplot3, fig.width=6, fig.height=3-------------------------------
sample.gr$color <- sample.int(6, length(SNP), replace=TRUE)
sample.gr$border <- sample(c("gray80", "gray30"), length(SNP), replace=TRUE)
lolliplot(sample.gr, features)

## ----lolliplot.index, fig.width=6, fig.height=3--------------------------
sample.gr$label <- as.character(1:length(sample.gr))
sample.gr$label.col <- "white"
lolliplot(sample.gr, features)

## ----lolliplot4, fig.width=6, fig.height=3-------------------------------
features$height <- c(0.02, 0.05, 0.08)
lolliplot(sample.gr, features)
## keep the height by giving the unit
features$height <- list(unit(1/16, "inches"), 
                     unit(3, "mm"), 
                     unit(12, "points"))
lolliplot(sample.gr, features)

## ----lolliplot.mul.features, fig.width=6, fig.height=3-------------------
features.mul <- rep(features, 2)
features.mul$height[4:6] <- list(unit(1/8, "inches"),
                                 unit(0.5, "lines"),
                                 unit(.2, "char"))
features.mul$fill <- c("#FF8833", "#F9712A", "#DFA32D", 
                       "#51C6E6", "#009DDA", "#4B9CDF")
end(features.mul)[5] <- end(features.mul[5])+50
features.mul$featureLayerID <- 
    paste("tx", rep(1:2, each=length(features)), sep="_")
names(features.mul) <- 
    paste(features.mul$featureLayerID, 
          rep(1:length(features), 2), sep="_")
lolliplot(sample.gr, features.mul)
## one name per transcripts
names(features.mul) <- features.mul$featureLayerID
lolliplot(sample.gr, features.mul)

## ----lolliplot4.1, fig.width=6, fig.height=3.5---------------------------
#Note: the score value is integer less than 10
sample.gr$score <- sample.int(5, length(sample.gr), replace = TRUE)
lolliplot(sample.gr, features)
##remove yaxis
lolliplot(sample.gr, features, yaxis=FALSE)

## ----lolliplot4.2, fig.width=6, fig.height=4.5---------------------------
#Try score value greater than 10
sample.gr$score <- sample.int(20, length(sample.gr), replace=TRUE)
lolliplot(sample.gr, features)
#Try float numeric score
sample.gr$score <- runif(length(sample.gr))*10
lolliplot(sample.gr, features)
# score should not be smaller than 1

## ----lolliplot.xaxis, fig.width=6, fig.height=4.5------------------------
xaxis <- c(1, 200, 400, 701, 1000, 1200, 1402) ## define the position
lolliplot(sample.gr, features, xaxis=xaxis)
names(xaxis) <- xaxis # define the labels
names(xaxis)[4] <- "center" 
lolliplot(sample.gr, features, xaxis=xaxis)

## ----lolliplot.yaxis, fig.width=6, fig.height=4.5------------------------
yaxis <- c(0, 5) ## define the position
lolliplot(sample.gr, features, yaxis=yaxis)
yaxis <- c(0, 5, 10, 15)
names(yaxis) <- yaxis # define the labels
names(yaxis)[3] <- "yaxis" 
lolliplot(sample.gr, features, yaxis=yaxis)

## ----lolliplot.jitter, fig.width=6, fig.height=4.5-----------------------
sample.gr$dashline.col <- sample.gr$color
lolliplot(sample.gr, features, jitter="label")

## ----lolliplot.legend, fig.width=6, fig.height=5-------------------------
legend <- 1:6 ## legend fill color
names(legend) <- paste0("legend", letters[1:6]) ## legend labels
lolliplot(sample.gr, features, legend=legend)
## use list to define more attributes. see ?grid::gpar to get more details.
legend <- list(labels=paste0("legend", LETTERS[1:6]), 
               col=palette()[6:1], 
               fill=palette()[legend])
lolliplot(sample.gr, features, legend=legend)
## if you have multiple tracks, try to set the legend by list.
## see more in section [Plot multiple samples](#plot-multiple-samples)
legend <- list(legend)
lolliplot(sample.gr, features, legend=legend)

## ----lolliplot.labels.control, fig.width=6, fig.height=5-----------------
sample.gr.rot <- sample.gr
sample.gr.rot$label.parameter.rot <- 45
lolliplot(sample.gr.rot, features, legend=legend)
sample.gr.rot$label.parameter.rot <- 60
sample.gr.rot$label.parameter.gp <- gpar(col="brown")
lolliplot(sample.gr.rot, features, legend=legend)

## ----lolliplot.xlab.ylab.title, fig.width=6, fig.height=5.2--------------
lolliplot(sample.gr.rot, features, legend=legend, ylab="y label here")
grid.text("x label here", x=.5, y=.01, just="bottom")
grid.text("title here", x=.5, y=.98, just="top", 
          gp=gpar(cex=1.5, fontface="bold"))

## ----lolliplot5, fig.width=6, fig.height=4.5-----------------------------
lolliplot(sample.gr, features, type="pin")
sample.gr$color <- lapply(sample.gr$color, function(.ele) c(.ele, sample.int(6, 1)))
sample.gr$border <- sample.int(6, length(SNP), replace=TRUE)
lolliplot(sample.gr, features, type="pin")

## ----lolliplot6, fig.width=6, fig.height=3-------------------------------
sample.gr$score <- NULL ## must be removed, because pie will consider all the numeric columns except column "color", "fill", "lwd", "id" and "id.col".
sample.gr$label <- NULL
sample.gr$label.col <- NULL
x <- sample.int(100, length(SNP))
sample.gr$value1 <- x
sample.gr$value2 <- 100 - x
## the length of color should be no less than the values number
sample.gr$color <- rep(list(c("#87CEFA", '#98CE31')), length(SNP))
sample.gr$border <- "gray30"
lolliplot(sample.gr, features, type="pie")

## ----lolliplot7, fig.width=6, fig.height=5.5-----------------------------
SNP2 <- sample(4000:8000, 30)
x2 <- sample.int(100, length(SNP2), replace=TRUE)
sample2.gr <- GRanges("chr3", IRanges(SNP2, width=1, names=paste0("snp", SNP2)), 
             value1=x2, value2=100-x2)
sample2.gr$color <- rep(list(c('#DB7575', '#FFD700')), length(SNP2))
sample2.gr$border <- "gray30"

features2 <- GRanges("chr3", IRanges(c(5001, 5801, 7001), 
                                    width=c(500, 500, 405),
                                    names=paste0("block", 4:6)),
                    fill=c("orange", "gray30", "lightblue"),
                    height=unit(c(0.5, 0.3, 0.8), "cm"))
legends <- list(list(labels=c("WT", "MUT"), fill=c("#87CEFA", '#98CE31')), 
                list(labels=c("WT", "MUT"), fill=c('#DB7575', '#FFD700')))
lolliplot(list(A=sample.gr, B=sample2.gr), 
          list(x=features, y=features2), 
          type="pie", legend=legends)

## ----lolliplot.multiple.type, fig.width=6, fig.height=7.5----------------
sample2.gr$score <- sample2.gr$value1 ## circle layout need score column 
lolliplot(list(A=sample.gr, B=sample2.gr), 
          list(x=features, y=features2), 
          type=c("pie", "circle"), legend=legends)

## ----lolliplot.pie.stack, fig.width=6, fig.height=5----------------------
rand.id <- sample.int(length(sample.gr), 3*length(sample.gr), replace=TRUE)
rand.id <- sort(rand.id)
sample.gr.mul.patient <- sample.gr[rand.id]
## pie.stack require metadata "stack.factor", and the metadata can not be 
## stack.factor.order or stack.factor.first
len.max <- max(table(rand.id))
stack.factors <- paste0("patient", formatC(1:len.max, 
                                           width=nchar(as.character(len.max)), 
                                           flag="0"))
sample.gr.mul.patient$stack.factor <- 
    unlist(lapply(table(rand.id), sample, x=stack.factors))
sample.gr.mul.patient$value1 <- 
    sample.int(100, length(sample.gr.mul.patient), replace=TRUE)
sample.gr.mul.patient$value2 <- 100 - sample.gr.mul.patient$value1
patient.color.set <- as.list(as.data.frame(rbind(rainbow(length(stack.factors)), 
                                                 "#FFFFFFFF"), 
                                           stringsAsFactors=FALSE))
names(patient.color.set) <- stack.factors
sample.gr.mul.patient$color <- 
    patient.color.set[sample.gr.mul.patient$stack.factor]
legend <- list(labels=stack.factors, col="gray80", 
               fill=sapply(patient.color.set, `[`, 1))
lolliplot(sample.gr.mul.patient, features, type="pie.stack", 
          legend=legend, dashline.col="gray")

## ----lolliplot.caterpillar, fig.width=6, fig.height=4--------------------
sample.gr$SNPsideID <- sample(c("top", "bottom"), 
                               length(sample.gr),
                               replace=TRUE)
lolliplot(sample.gr, features, type="pie", 
          legend=legends[[1]])

## ----lolliplot.caterpillar2, fig.width=6, fig.height=12------------------
## two layers
sample2.gr$SNPsideID <- "top"
idx <- sample.int(length(sample2.gr), 15)
sample2.gr$SNPsideID[idx] <- "bottom"
sample2.gr$color[idx] <- '#FFD700'
lolliplot(list(A=sample.gr, B=sample2.gr), 
          list(x=features.mul, y=features2), 
          type=c("pie", "circle"), legend=legends)

## ----VCF, fig.width=6, fig.height=5--------------------------------------
library(VariantAnnotation)
library(TxDb.Hsapiens.UCSC.hg19.knownGene)
library(org.Hs.eg.db)
fl <- system.file("extdata", "chr22.vcf.gz", package="VariantAnnotation")
gr <- GRanges("22", IRanges(50968014, 50970514, names="TYMP"))
tab <- TabixFile(fl)
vcf <- readVcf(fl, "hg19", param=gr)
mutation.frequency <- rowRanges(vcf)
mcols(mutation.frequency) <- cbind(mcols(mutation.frequency), 
                                   VariantAnnotation::info(vcf))
mutation.frequency$border <- "gray30"
mutation.frequency$color <- 
    ifelse(grepl("^rs", names(mutation.frequency)), "lightcyan", "lavender")
## plot Global Allele Frequency based on AC/AN
mutation.frequency$score <- mutation.frequency$AF*100
seqlevelsStyle(gr) <- seqlevelsStyle(mutation.frequency) <- "UCSC" 
trs <- geneModelFromTxdb(TxDb.Hsapiens.UCSC.hg19.knownGene,
                         org.Hs.eg.db,
                         gr=gr)
features <- c(range(trs[[1]]$dat), range(trs[[5]]$dat))
names(features) <- c(trs[[1]]$name, trs[[5]]$name)
features$fill <- c("lightblue", "mistyrose")
features$height <- c(.02, .04)
lolliplot(mutation.frequency, features, ranges=gr)

## ----methylation, eval=FALSE, echo=TRUE----------------------------------
#  library(rtracklayer)
#  session <- browserSession()
#  query <- ucscTableQuery(session, track="HAIB Methyl RRBS",
#                          range=GRangesForUCSCGenome("hg19", seqnames(gr), ranges(gr)))
#  tableName(query) <- tableNames(query)[1]
#  methy <- track(query)
#  methy <- GRanges(methy)

## ----methylation.hide, echo=FALSE----------------------------------------
methy <- import(system.file("extdata", "methy.bed", package="trackViewer"), "BED")

## ----fig.width=6,fig.height=4--------------------------------------------
lolliplot(methy, features, ranges=gr, type="pin")

## ----fig.width=6,fig.height=2.5------------------------------------------
methy$lwd <- .5
lolliplot(methy, features, ranges=gr, type="pin", cex=.5)
lolliplot(methy, features, ranges=gr, type="circle", cex=.5)
methy$score2 <- max(methy$score) - methy$score
lolliplot(methy, features, ranges=gr, type="pie", cex=.5)
## we can change it one by one
methy$cex <- runif(length(methy))
lolliplot(methy, features, ranges=gr, type="pin")
lolliplot(methy, features, ranges=gr, type="circle")

## ----fig.width=4.5,fig.height=3------------------------------------------
dandelion.plot(methy, features, ranges=gr, type="pin")

## ----fig.width=4.5,fig.height=3------------------------------------------
methy$color <- 3
methy$border <- "gray"
## score info is required, the score must be a number in [0, 1]
m <- max(methy$score)
methy$score <- methy$score/m
dandelion.plot(methy, features, ranges=gr, type="fan")

## ----fig.width=4.5,fig.height=4------------------------------------------
methy$color <- rep(list(c(3, 5)), length(methy))
methy$score2 <- methy$score2/m
legends <- list(list(labels=c("s1", "s2"), fill=c(3, 5)))
dandelion.plot(methy, features, ranges=gr, type="pie", legend=legends)

## ----fig.width=4.5,fig.height=2.5----------------------------------------
## want less
dandelion.plot(methy, features, ranges=gr, type="circle", maxgaps=1/10)

## ----fig.width=4.5,fig.height=4.5----------------------------------------
## want more
dandelion.plot(methy, features, ranges=gr, type="circle", maxgaps=1/100)

## ----eval=FALSE----------------------------------------------------------
#  ideo <- loadIdeogram("hg38")

## ----echo=FALSE, results="hide"------------------------------------------
path <- system.file("extdata", "ideo.hg38.rds", package = "trackViewer")
ideo <- readRDS(path)

## ------------------------------------------------------------------------
dataList <- ideo
dataList$score <- as.numeric(dataList$gieStain)
dataList <- dataList[dataList$gieStain!="gneg"]
dataList <- GRangesList(dataList)
ideogramPlot(ideo, dataList, 
            layout=list("chr1", c("chr3", "chr22"), 
                        c("chr4", "chr21")))

## ----sessionInfo, results='asis'-----------------------------------------
sessionInfo()